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2DE Protein Digestion Capillary Electrophoresis Sample preparation Protein Extraction List [total 7] 1. Protocol Online http://www.protocol-online.org/ 2. 2D PAGE Western Blotting and ECL Detection 2D PAGE Western Blotting and ECL Detection [1, 2, see also Amersham ECL Kit instructions]  Solutions 1. 10x TBS, pH 7.6 (Tris-buffered saline) stock. To make 2 liters, add 48.4 g Tris base (0.... 3. Trypsin in-gel digestion of proteins Trypsin in-gel digestion of proteins(adapted from Shevchenko et al. Anal. Chem. 68, pp. 850-858, 1996)1. Excise protein spot/band and dehydrate in CH3CN for approx. 10 min. Remove CH3CN and SpeedVa... 4. Sample preparation Sample SolutionSample Buffer ReagentsAmount7 M Urea21 g dry urea2 M Thiourea7.6 g100 mM DTE770 mg4 % CHAPS2 g0.5 % Carrier ampholytes250 μl (pharmalytes)40 mM tris240 mg0.002 % Bromophenol Blue dy... 5. ISB\'s protocols http://www.proteomecenter.org/protocols.php 6. OpenWetWare OpenWetWare is an effort to promote the sharing of information, know-how, and wisdom among researchers and groups who are working in biology & biological engineering. OpenWetWare, was set up ju... http://openwetware.org/wiki/Main_Page Copyright (c) 2010 Yonsei Proteome Research Center